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anti gnas (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti gnas
Anti Gnas, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gnas/pmc12892574-72-10-19
Average 86 stars, based on 1 article reviews

anti gnas - by Bioz Stars, 2026-09

86/100 stars

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Article Title: Activation of the pituitary adenylate cyclase-activating polypeptide type I receptor promotes neuroblastoma proliferation and migration through distinct G protein pathways
Article Snippet: Anti-ERK (#9102), anti-phospho-ERK (Thr202/Tyr204) (#4370), anti-AKT (#4691), anti-phospho-AKT (T308) (#9275), anti-GNAS (#47342), and anti-CyclinD1 (#55506) antibodies were purchased from Cell Signaling Technology.

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( A ) GO analysis of DEG between clusters 5 and 8. ( B ) Heatmap of DEG in clusters 5 and 8. ( C ) Feature plot showing the expression of <t>Gnas</t> in mesenchymal and tendon lineage cells. ( D ) Representative immunofluorescence images of GNAS expression in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi. Scale bar, 50 μm. ( E , F ) Statistical analysis of GNAS + region ( E ) and GNAS + /GLI1 + region ( F ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( E ): 7 dpi vs uninjured **** P = 9.47e-11. 21 dpi vs uninjured **** P = 2.03e-8, 63 dpi vs uninjured **** P = 3.21e-5. P values in ( F ): 7 dpi vs uninjured **** P = 2.64e-6, 21 dpi vs uninjured **** P = 3.64e-6, 63 dpi vs uninjured **** P = 9.58e-5. ( G, H ) Statistical analysis of p-PKA substrate + region ( G ) and p-PKA substrate + /GLI1 + region ( H ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( G ): 7 dpi vs uninjured **** P = 6.33e-6, 21 dpi vs uninjured *** P = 1.15e-4, 63 dpi vs uninjured * P = 1.17e-2. P values in ( H ): 7 dpi vs uninjured **** P = 1.96e-5, 21 dpi vs uninjured *** P = 2.63e-3, 63 dpi vs uninjured **** P = 6.44e-4. ( I – L ) Representative immunofluorescence images of p-PKA substrate expression in uninjured tendon ( I ) and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7 ( J ), 21 ( K ), and 63 dpi ( L ). Scale bar, 50 μm. Data are presented as mean ± SD. All P values were determined by unpaired Student’s t test.

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( A ) GO analysis of DEG between clusters 5 and 8. ( B ) Heatmap of DEG in clusters 5 and 8. ( C ) Feature plot showing the expression of Gnas in mesenchymal and tendon lineage cells. ( D ) Representative immunofluorescence images of GNAS expression in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi. Scale bar, 50 μm. ( E , F ) Statistical analysis of GNAS + region ( E ) and GNAS + /GLI1 + region ( F ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( E ): 7 dpi vs uninjured **** P = 9.47e-11. 21 dpi vs uninjured **** P = 2.03e-8, 63 dpi vs uninjured **** P = 3.21e-5. P values in ( F ): 7 dpi vs uninjured **** P = 2.64e-6, 21 dpi vs uninjured **** P = 3.64e-6, 63 dpi vs uninjured **** P = 9.58e-5. ( G, H ) Statistical analysis of p-PKA substrate + region ( G ) and p-PKA substrate + /GLI1 + region ( H ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( G ): 7 dpi vs uninjured **** P = 6.33e-6, 21 dpi vs uninjured *** P = 1.15e-4, 63 dpi vs uninjured * P = 1.17e-2. P values in ( H ): 7 dpi vs uninjured **** P = 1.96e-5, 21 dpi vs uninjured *** P = 2.63e-3, 63 dpi vs uninjured **** P = 6.44e-4. ( I – L ) Representative immunofluorescence images of p-PKA substrate expression in uninjured tendon ( I ) and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7 ( J ), 21 ( K ), and 63 dpi ( L ). Scale bar, 50 μm. Data are presented as mean ± SD. All P values were determined by unpaired Student’s t test.

Journal: The EMBO Journal

Article Title: GNAS/PKA signaling promotes aberrant osteochondral differentiation of Gli1 + tendon sheath progenitors

doi: 10.1038/s44318-025-00553-7

Figure Lengend Snippet: ( A ) GO analysis of DEG between clusters 5 and 8. ( B ) Heatmap of DEG in clusters 5 and 8. ( C ) Feature plot showing the expression of Gnas in mesenchymal and tendon lineage cells. ( D ) Representative immunofluorescence images of GNAS expression in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi. Scale bar, 50 μm. ( E , F ) Statistical analysis of GNAS + region ( E ) and GNAS + /GLI1 + region ( F ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( E ): 7 dpi vs uninjured **** P = 9.47e-11. 21 dpi vs uninjured **** P = 2.03e-8, 63 dpi vs uninjured **** P = 3.21e-5. P values in ( F ): 7 dpi vs uninjured **** P = 2.64e-6, 21 dpi vs uninjured **** P = 3.64e-6, 63 dpi vs uninjured **** P = 9.58e-5. ( G, H ) Statistical analysis of p-PKA substrate + region ( G ) and p-PKA substrate + /GLI1 + region ( H ) in uninjured tendon and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7, 21, and 63 dpi ( n = 5 per group). P values in ( G ): 7 dpi vs uninjured **** P = 6.33e-6, 21 dpi vs uninjured *** P = 1.15e-4, 63 dpi vs uninjured * P = 1.17e-2. P values in ( H ): 7 dpi vs uninjured **** P = 1.96e-5, 21 dpi vs uninjured *** P = 2.63e-3, 63 dpi vs uninjured **** P = 6.44e-4. ( I – L ) Representative immunofluorescence images of p-PKA substrate expression in uninjured tendon ( I ) and injured site of Gli1 - Cre ERT2 ; Ai9 mice at 7 ( J ), 21 ( K ), and 63 dpi ( L ). Scale bar, 50 μm. Data are presented as mean ± SD. All P values were determined by unpaired Student’s t test.

Article Snippet: Anti-mouse GNAS , Proteintech , Cat#10150-2-AP.

Techniques: Expressing, Immunofluorescence

( A – D ) Representative immunofluorescence images of GNAS expression in uninjured tendons ( A ) and injured sites of Gli1 - CreER T2 ; Ai9 mice at 5 ( B ), 7 ( C ) and 14 ( D ) dpi. Scale bar, 50 μm. ( E , F ) Statistical analysis of GNAS + region ( E ) and GNAS + /GLI1 + region ( F ) in uninjured tendons and injured sites of Gli1 - CreER T2 ; Acvr1 R206H/+ mice at 5, 7 and 14 dpi ( n = 5 per group). P values from left to right: **** P = 8.29e-10, **** P = 1.16e-8, **** P = 1.57e-8. **** P = 2.36e-9, **** P = 1.71e-6, **** P = 9.06e-6. ( G , H ) Statistical analysis of p-PKA substrate + region ( G ) and p-PKA substrate + /GLI1 + region ( H ) in uninjured tendons and injured sites of Gli1 - CreER T2 ; Acvr1 R206H/+ mice at 5, 7 and 14 dpi ( n = 5 per group). P values from left to right: **** P = 1.42e-6, **** P = 7.23e-6, **** P = 1.53e-5. **** P = 1.96e-5, ** P = 2.63e-3, **** P = 6.44e-4. ( I – L ) Representative immunofluorescence images of p-PKA substrate expression in uninjured tendons ( I ) and injured sites of Gli1 - CreER T2 ; Acvr1 R206H/+ mice at 5 ( J ), 7 ( K ) and 14 ( L ) dpi. Scale bar, 50 μm. Data is presented as mean ± SD. All P values were determined by unpaired Student’s t test.

Journal: The EMBO Journal

Article Title: GNAS/PKA signaling promotes aberrant osteochondral differentiation of Gli1 + tendon sheath progenitors

doi: 10.1038/s44318-025-00553-7

Figure Lengend Snippet: ( A – D ) Representative immunofluorescence images of GNAS expression in uninjured tendons ( A ) and injured sites of Gli1 - CreER T2 ; Ai9 mice at 5 ( B ), 7 ( C ) and 14 ( D ) dpi. Scale bar, 50 μm. ( E , F ) Statistical analysis of GNAS + region ( E ) and GNAS + /GLI1 + region ( F ) in uninjured tendons and injured sites of Gli1 - CreER T2 ; Acvr1 R206H/+ mice at 5, 7 and 14 dpi ( n = 5 per group). P values from left to right: **** P = 8.29e-10, **** P = 1.16e-8, **** P = 1.57e-8. **** P = 2.36e-9, **** P = 1.71e-6, **** P = 9.06e-6. ( G , H ) Statistical analysis of p-PKA substrate + region ( G ) and p-PKA substrate + /GLI1 + region ( H ) in uninjured tendons and injured sites of Gli1 - CreER T2 ; Acvr1 R206H/+ mice at 5, 7 and 14 dpi ( n = 5 per group). P values from left to right: **** P = 1.42e-6, **** P = 7.23e-6, **** P = 1.53e-5. **** P = 1.96e-5, ** P = 2.63e-3, **** P = 6.44e-4. ( I – L ) Representative immunofluorescence images of p-PKA substrate expression in uninjured tendons ( I ) and injured sites of Gli1 - CreER T2 ; Acvr1 R206H/+ mice at 5 ( J ), 7 ( K ) and 14 ( L ) dpi. Scale bar, 50 μm. Data is presented as mean ± SD. All P values were determined by unpaired Student’s t test.

Article Snippet: Anti-mouse GNAS , Proteintech , Cat#10150-2-AP.

Techniques: Immunofluorescence, Expressing

( A ) Representative HE staining showing the morphology of human tendon. Scale bar, 200 or 50 μm. ( B ) Representative immunofluorescence staining of GLI1 in human tendon. Scale bar, 10 μm. ( C ) Representative HE staining showing the morphology of HO from Ossification of the Posterior Longitudinal Ligament patients. Scale bar, 200 or 50 μm. ( D ) Representative immunofluorescence staining of GLI1 in HO from Ossification of the Posterior Longitudinal Ligament patients. Scale bar, 10 μm. ( E ) Statistical analysis of the GLI1 + cells in human normal tendon and HO lesion ( n = 3 per group). **** P = 7.88e-7. ( F , G ) Representative immunofluorescence staining ( F ) and statistical analysis ( G ) of GNAS in naive or chondrogenic tendon stem cells with or without NF449 treatment. ( n = 5 per group). **** P = 2.8e-14. Scale bar, 10 μm. ( H , I ) Representative immunofluorescence staining ( H ) and statistical analysis ( I ) of p-PKA substrate in naive or chondrogenic tendon stem cells with or without NF449 treatment. ( n = 5 per group). Data are represented as the mean ± SD. **** P = 9.77e-9. Scale bar, 10 μm. ( J , K ) Statistical analysis of SOX9 ( J , **** P = 8.91e-8) and RUNX2 ( K , **** P = 6.62e-6) in chondrogenic or osteogenic tendon stem cells with or without NF449 treatment. ( n = 6 per group). Data is presented as mean ± SD. All P values were determined by unpaired Student’s t test ( E ) and one-way ANOVA with Bonferroni post hoc test ( G , I , J , K ).

Journal: The EMBO Journal

Article Title: GNAS/PKA signaling promotes aberrant osteochondral differentiation of Gli1 + tendon sheath progenitors

doi: 10.1038/s44318-025-00553-7

Figure Lengend Snippet: ( A ) Representative HE staining showing the morphology of human tendon. Scale bar, 200 or 50 μm. ( B ) Representative immunofluorescence staining of GLI1 in human tendon. Scale bar, 10 μm. ( C ) Representative HE staining showing the morphology of HO from Ossification of the Posterior Longitudinal Ligament patients. Scale bar, 200 or 50 μm. ( D ) Representative immunofluorescence staining of GLI1 in HO from Ossification of the Posterior Longitudinal Ligament patients. Scale bar, 10 μm. ( E ) Statistical analysis of the GLI1 + cells in human normal tendon and HO lesion ( n = 3 per group). **** P = 7.88e-7. ( F , G ) Representative immunofluorescence staining ( F ) and statistical analysis ( G ) of GNAS in naive or chondrogenic tendon stem cells with or without NF449 treatment. ( n = 5 per group). **** P = 2.8e-14. Scale bar, 10 μm. ( H , I ) Representative immunofluorescence staining ( H ) and statistical analysis ( I ) of p-PKA substrate in naive or chondrogenic tendon stem cells with or without NF449 treatment. ( n = 5 per group). Data are represented as the mean ± SD. **** P = 9.77e-9. Scale bar, 10 μm. ( J , K ) Statistical analysis of SOX9 ( J , **** P = 8.91e-8) and RUNX2 ( K , **** P = 6.62e-6) in chondrogenic or osteogenic tendon stem cells with or without NF449 treatment. ( n = 6 per group). Data is presented as mean ± SD. All P values were determined by unpaired Student’s t test ( E ) and one-way ANOVA with Bonferroni post hoc test ( G , I , J , K ).

Article Snippet: Anti-mouse GNAS , Proteintech , Cat#10150-2-AP.

Techniques: Staining, Immunofluorescence

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